Support. Find everything you need to start setting up your lab, including special savings, checklists, and more From scientific discovery to scale-up and commercial delivery, Avantor offers mission-critical products, services and solutions on a global scale. For more information, call 1.888.793.2300. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5. Formula Weight: 238.30 CAS No. Methods J. PubMedGoogle Scholar. Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. PubMed et al. : Derivative potentiometric stripping analysis of Cu(II) at nanomolar levels exploiting the 4-[10,15,20-tris(4-sulfophenyl)-21,22-dihydroporphyrin-5-yl] benzenesulfonic acid host compound on multi-walled carbon nanotubes. 2006. ,Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate Learn more . Contaminants: chloride: <0.01%; sulfate: <0.05%. C8H17N2NaO4S, Sol. Clin. Cleanrooms and controlled environments are used for ultraclean conditions in research and manufacturing. Your session has expired. Supplier: VWR. Some documentation and label information may refer to the legacy brand. HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. Chem. Explore more media supplements Additional media supplements Cholesterol supplements Insulin It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. HEPES sodium salt solution is suitable for use in cell culture studies. Water Res. J. Chem. Biol. Registration No 3,257,926) Please contact your purchasing department for more information. You can select and customize services for peak efficiency, quality, and accelerated innovation. Copyright 2007-2023 Santa Cruz Biotechnology Inc. All Rights Reserved. Good set forth several criteria for such buffers: A pKa between 6 and 8. 1966. Avantor can help equip your life sciences lab with the products, equipment, and supplies you need whether you work in cell biology, genomics, proteomics, or other fields. Arab. J Solution Chem 50, 147159 (2021). Cliquez sur le lien pour afficher lentre PubMed casNumber.value : '' }}, $12.99 Ground shipping (In continental US only.). https://doi.org/10.1038/srep37672, Article This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Solution Chem. J Neurosci. These goggles are comfortable and have good peripheral vision. currentSelection.sku : '' }}, {{ casNumber && casNumber.value ? Avantor Services provides a wide range of specialized services and digital solutions to help you solve complex challenges. HEPES Sodium salt | CAS 75277-39-3 SAMPLE Certificate of Analysis. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Our product offerings can help with biomedical research, forensic work, and clinical diagnostics. Meth. New Avantor J.T.Baker premium conductive and non-conductive robotic tips deliver superior quality and reliable performance for results you can trust. 7.5. During the process of. |Zarnowska, ED. Avantor has the resources to make your Chromatography or Mass Spectrometry applications run efficiently and effectivelyfrom the measuring apparatus needed for chromatography, or the proteins used to fulfill sample manipulation during mass spectrometry. Biochemistry. Durable, low-linting, & available in white or blue. Avantor supports end-to-end fluid management solutions including peristaltic pumps and aseptic fluid transfer solutions that are reliable and customer-centric, helping bioprocessing manufacturers meet their research and production goals. Provided by the Springer Nature SharedIt content-sharing initiative, Determination of Solubility of 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic Acid and its Sodium Salt in Acetonitrile and Voltammetric Investigation of Sulphonamide Drugs in Different Solvents in Their Absence and Presence, https://doi.org/10.1007/s10953-020-01047-2, 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, Solubility Determination of Hydroquinone in Dichloromethane, Trichloromethane and Carbon Tetrachloride by Using the Co-solvent Calibration Method, Effect of monomer structure of anionic surfactant on voltammetric signals of an anticancer drug: rapid, simple, and sensitive electroanalysis of nilotinib in biological samples, Determination of Dissociation Constants of (5,6-Dioxo-3-sulfoquinoline-7-yloxy)acetic Acid in Aqueous Solution at 25C by Potentiometric Titration, The Reaction of 2-Chloroquinoxaline with Piperidine in DMSOH2O and DMFH2O Mixtures: Kinetics and Solvent Effects, Determination of the ionization constants of sulfonylureas in THFwater media by potentiometric titration and RPLC methods, Behavior of Antibacterial Ofloxacin; Hydronation Constants and Solubility in Aqueous Solutions of Sodium Chloride at Different Temperatures, Solubility and Dissolution Thermodynamics of Cefmetazole Acid in Four Neat Solvents and Preferential Solvation in Co-Solvent Mixtures of (Methanol, Ethanol or Isopropanol)+Water, Voltammetric study of valsartanNi complex: application to valsartan analysis in pharmaceuticals and in vivo human urine profiling, Comparative Study on the Oxidation Kinetics of Structural Isomers by Acidified N-Bromo-Benzenesulfonamide: A Mechanistic Determination, http://creativecommons.org/licenses/by/4.0/. References: Li, W. and Schlessinger, Mol. Its applications include, tissue culture, Phosphorylation and photophosphorylation, fixative Transmission electron microscopy, protein synthesis, preventing binding to non-receptor materials. 1971. Binders, calendars, pens, cleaning and sanitation supplies, and office equipment are just some of the essential products we offer Science education supplies, specimens, activities, and equipment for all grade levels kindergarten to college. PMID: 16531090 | Role of interleukin-4 and prostaglandin E2 in Leishmania amazonensis infection of BALB/c mice. Multi purpose buffer used in biological research. To obtain a range of pH values, HEPES- and PIPES-buffered media were . 21, 1475 (2009), Lahcen, A.A., Errayess, S.A., Amine, A.: Voltammetric determination of sulfonamides using paste electrodes based on various carbon nanomaterials. Product Description: HEPES, sodium salt: HEPES, sodium salt. et al. HEPES-buffered saline (HBS) solution: Prepare 2 HBS stock by adding the following to 400 ml dH 2 O: 50 ml 1 M HEPES, 28 ml 5 M NaCl and 1.5 ml 0.5 M Na 2 HPO 4. We continue to set science in motion to create a better world by providing you with the right solutions to keep moving forward. Your email subscription to News & Announcements has been received. Compare this item SIGMA HEPES sodium salt, =99.5% (titration) MilliporeSigma Synonyms: As it could be demonstrated the two non-aqueous solvents together provided results which completed each other providing useful information about studied systems. To process your orders without delay, please provide the required business documentation to purchase this product. Lszl Kiss. 8: 1219-26. Solubility. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 8.2. Has a pKa of 7.48 at 25C. In Vitro Cell. Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve. 14, 6150 (2017), Zigler, J.S., Lepe-Zuniga, J.L., Vistica, B., Gary, I.: Analysis of the cytotoxic effects of light-exposed hepes-containing culture medium. 2015. Unsure about this product? Sub-category not found for Other Products category. Environ. Talanta 39, 631 (1992), Carrazon, J.M.P., Corona, P.C., Diez, L.M.P. PMID: 4206745 4. hepes may be the free acid whereas the sodium salt is neutralized. 3, 56 (2010), Fogg, A.G., Rahim, H.A., Yusoff, M., Moreira, J.C., Chao, R.: Cathodic stripping voltammetric determination of sulfonamides as copper(I) complexes at a hanging mercury drop electrode. You have the right to unsubscribe at any time by clicking the 'unsubscribe' link in any News & Announcement emails you receive. L1 sulphadiazine in dimethyl sulphoxide, Normalized fluorescence intensities of sulphonamide drugs in presence of HEPES and its sodium salt. bvseo-msg: The resource to the URL or file is currently unavailable. We produce an extensive selection of cell culture media supplements to support of the growth and maintenance of your cells. Choose Gibco media supplements to customize your cell culture. 86, 4416 (2014), Pimentel, D.M., Silva, S.M., Leite, F.R.F., Tanaka, A.A., Santos, W.T.P., Damos, F.S., Luz, R.C.S. 491, 175 (2000), Braga, O.C., Campestrini, I., Vieira, I.C., Spinelli, A.: Sulfadiazine determination in pharmaceuticals by electrochemical reduction on a glassy carbon electrode. VAI's cellulose free, low particlate and chemical resistant cleanroom paper. Acta 159, 119 (1984), Article HEPES [N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)] is a general purpose buffer for biological research. The sodium salt of HEPES is more soluble than it's free acid counterpart. ACS Chem. 1990. PMID: 16531090 | Role of interleukin-4 and prostaglandin E2 in Leishmania amazonensis infection of BALB/c mice. Innovation in battery technology is becoming vital to facilitate the energy transition. 25: 909-913. It may be used in the preparation of buffer solution of HEPES. HEPES sodium salt solution (1M) is a zwitterionic buffer used to maintain pH of solutions. Gold Biotechnology (U.S. Google Scholar, Nikoofard, H., Sargolzaei, M., Faridbod, F.: Prediction of physico-chemical properties of bacteriostatic N1-substituted sulfonamides: theoretical and experimental studies. 182: 50-68. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. Meth. HEPES is used in many media because it has more buffering capacity than sodium bicarbonate at physiological pH (7.2 - 7.4) at 37C. As our customers needs have evolved, so have our capabilities. Asked 5th Sep, 2018 Prasun Kumar University of Bristol How to prepare HEPES Sodium buffer from HEPES? The sodium salt of HEPES is more soluble than it's free acid counterpart. Avantor helps keep you safe with a robust line of safety products and personal protective equipment -- from waste bins to safety signs. Cell . Used under license. PubMed Central Get trusted safety products from Avantor. Santa Cruz Biotechnology, and the Santa Cruz Biotechnology, Inc. logo, Santa Cruz Animal Health, San Juan Ranch, Supplement of Champions, the San Juan Ranch logo, UltraCruz, ChemCruz, ImmunoCruz, ExactaCruz, and EZ Touch are registered trademarks of Santa Cruz Biotechnology, Inc. All trademarks are the property of their respective owners. No. Binders, calendars, pens, cleaning and sanitation supplies, and office equipment are just some of the essential products we offer Science education supplies, specimens, activities, and equipment for all grade levels kindergarten to college. JavaScript seems to be disabled in your browser. What is HEPES buffer used for? HEPES sodium salt solution (1M) is prepared with high-quality reagents and 0.2 m filtered; however it has not been evaluated for use in cell culture. Correspondence to Avantor supports end-to-end fluid management solutions including Masterflex peristaltic pumps and aseptic fluid transfer solutions that are reliable and customer-centric, helping bioprocessing manufacturers meet their research and production goals. 17: 651-653. The material represented by this grade is not suitable to be used as an Excipient, Active Pharmaceutical Ingredient, Drug, Drug Product or household item. 75277-39-3, Formula: Available in 8.5x11, A4, pre-hole punched and multiple colors. 1990. Used under license. 52, 560 (2018), Article The original product is no longer available. Freeze cells in tubes from 1 to 5mL using the Thermo Scientific Mr. Frosty Freezing Container at nearly -1C/minute. Biol. tech@biobasic.com, 20 Konrad Crescent, Markham ON, Canada, L3R 8T4 | 4160 Bailey Avenue, Amherst, NY, USA, 14226 | Copyright 2017 Bio Basic Inc. All rights reserved. HEPES sodium salt, ULTROL. Clin. By closing this message, you consent to our, Hello, {{ user.first_name }} {{ user.last_name }}, Z')" data-type="collection" title="Products A->Z" target="_self" href="/collection/products-a-to-z">Products A->Z. We use operational excellence to deliver solutions that enable research, testing, production, and commercialization across the globe. Nothing that happens in the labor other workplace is as important as your health and safety. Product Description Molecular Formula: C8H17N2NaO4S Molecular Weight: 260.3 CAS Number: 75277-39-3 pKa12 = 3 pKa2 = 7.85 (0 C); 7.55 (20 C); 7.31 (37 C)2 pK/T3 = -0.014/ C Synonums: sodium N-(2-hydroxyethyl) piperazine-N'- HEPES ( 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. Google Scholar, Santos, T.A.D., Neto, S.Y., Macena, C.S., Luz, R.C.S., Damos, F.S. These goggles are comfortable and have good peripheral vision. HEPES sodium salt solution (1M) is a zwitterionic buffer used to maintain pH of solutions. The tendency of this buffer to form radicals makes it unsuitable for redox studies. Registration No 3,257,927) and Goldbio (U.S. and Izawa, S. 1972. Good, N.E., et al. Some documentation and label information may refer to the legacy brand. HEPES sodium salt solution is suitable for cell culture studies. To place an order using RMB or to ship to mainland China, please visit www.scbio.cn, Recommended working concentration: 10-25 mM, Anticorps secondaires monoclonaux et recombinants, PROTINE A, G-PLUS & L AGAROSE / REACTIFS IP, CRUZ MARKER Marqueurs de Poids Molculaire, Marqueurs de Poids Molculaire Prcolors, Ractifs de blocage pour des applications de Western Blot, PROTINES BIOLOGIQUEMENT ACTIVES & PURIFIES, Racclments de cytomtrie de flux (FCM), EXTRAITS TISSULAIRES & LYSATS CELLULAIRES, CELLULES SOUCHES & RACTIFS DE CULTURE CELLULAIRES, NUCLOTIDES, PEPTIDES, PROTINES & ACIDES AMINS, Protines & Peptides Biologiquement Actives, MARQUEURS, COLORANTS, SONDES & TIQUETTES, FILM AUTORADIOGRAPHIQUE, TIQUETTE ET FILM PLASTIQUE, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt; N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid) sodium salt. 5: 467-477. Enzymol. Avantor supports end-to-end fluid management solutions including peristaltic pumps and aseptic fluid transfer solutions that are reliable and customer-centric, helping bioprocessing manufacturers meet their research and production goals. VWR supports you in your autosampler vial selection process through on-site consultations with our Chromatography Specialists, and providing samples when needed. Financial support of the GINOP 2.3.2-15-2016-00022 Grant is highly appreciated. Innovation in battery technology is becoming vital to facilitate the energy transition. HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It contains a 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate. Our global footprint enables us to serve more than 300,000 customer locations and gives us extensive access to research laboratories and scientists in more than 180 countries. Contact VWR Custom Manufacturing Services at 1.800.932.5000 or VWRCustom@vwr.com. We have become experts in scientific operations, improving performance with sophisticated solutions and providing guidance on best practices. ; loc_en_US, sid_300789, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]. The tendency of this buffer to form radicals makes it unsuitable for redox studies. The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers. Hi All: One of my crystallisation condition contains HEPES-Sodium. Its applications include, tissue culture, Phosphorylation and photophosphorylation, fixative Transmission electron microscopy, protein synthesis, preventing binding to non-receptor materials. VWR provides the cell culture community with access to the most reliable supply of exceptional quality Fetal Bovine Serum: VWR Life Science Seradigm. Medzon, E.L. and Gedies, A. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5. and Izawa, S. 1972. Sodium chloride is reduced to keep the milliosmolality of the media containing both bicarbonate and HEPES in the range of non-HEPES containing media. As your trusted, collaborative partner, Avantor Services helps you increase efficiency, maximize productivity, and accelerate innovation. If you need further assistance, please call VWR Customer Service at 1-800-932-5000. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Weve built our reputation on consistent, comprehensive mastery of day-to-day operations, allowing lab, clinical, and production environments to focus their high-value resources on core scientific priorities. VWR is your complete source for workplace supplies. Acceptable forms of ID are: * ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. The electrochemical investigations showed that an acid-base reaction takes place between the outlined sulphonamide drugs and sodium salt of HEPES which acts as a basic component. : The oxidative voltammetric behaviour of some sulphonamides at the glassy carbon electrode. Please enable it to get the best site experience. Please employ appropriate sterilization techniques for the intended application. 2023 FORTUNE Media IP Limited All rights reserved. Medzon, E.L., et al. J. Braz. Its applications include, tissue culture, Phosphorylation and photophosphorylation, fixative Transmission electron microscopy, protein synthesis, preventing binding to non-receptor materials. J. Microbiol. Thank you for your question VAI's cellulose free, low particlate and chemical resistant cleanroom paper. HEPES Sodium Salt is a zwitterionic salt which is used in the preparation of a HEPES buffer solution. J. Electroanal. : Electroanalytical study of sulphamerazine at a glassy-carbon electrode and its determination in pharmaceutical preparations by HPLC with amperometric detection. Dependable -80C storage with a focus on sustainability without sacrificing sample protection. |Guimares, ET. Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. CAS #:75277-39-3 Molecular Formula: C Proc. Chem. PMID: 4206745 4. hepes sodium salt, hepes hemisodium salt, sodium 2-4-2-hydroxyethyl piperazin-1-yl ethanesulfonate, unii-z9fto91o8a, 1-piperazineethanesulfonic acid, 4-2-hydroxyethyl-, monosodium salt, z9fto91o8a, sodium 4-2-hydroxyethyl piperazin-1-ylethanesulphonate, 4-2-hydroxyethyl piperazine-1-ethanesulfonic acid sodium salt, 4-2-hydroxyethyl piperazine-1 . A strong, vibrant research and development group is the lifeblood of all industries. Google Scholar, Aeppli, M., Voegelin, A., Gorski, C.A., Hofstetter, T.B., Sander, M.: Mediated electrochemical reduction of iron (oxyhydr-)oxides under defined thermodynamic boundary conditions. High-quality chemicals and services, customized to your product or manufacturing needs VWR enables the advancement of science by providing high-quality chemicals and services, customized to your product or manufacturing needs. Determination of Solubility of 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic Acid and its Sodium Salt in Acetonitrile and Voltammetric Investigation of Sulphonamide Drugs in Different Solvents in Their Absence and Presence. 2023 VWR International, LLC. Hygroscopic - protect from moisture. A strong, vibrant research and development group is the lifeblood of all industries. For information visit, www.avantorsciences.com and find us on LinkedIn, Twitter and Facebook. 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Sci. Our product offerings can help with biomedical research, forensic work, and clinical diagnostics. New Avantor J.T.Baker premium conductive and non-conductive robotic tips deliver superior quality and reliable performance for results you can trust. 1971. Santa Cruz Biotechnology, Inc. est leader mondial dans le dveloppement de produits pour la recherche biomdicale. Freeze cells in tubes from 1 to 5mL using the Thermo Scientific Mr. Frosty Freezing Container at nearly -1C/minute. Contact VWR Custom Manufacturing Services at 1.800.932.5000 or VWRCustom@vwr.com. Avantor can help you plan, design, and configure a custom laboratory environment that meets all your needs. White solid. 20C: HEPES sodium salt, 99%. By signing up, you indicate that you have read and agree to the terms in our privacy policy. Contactez-nous au 1-800-457-3801. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay. HEPES [N- (2-hydroxyethyl)piperazine-N- (2-ethanesulfonic acid)] is a general purpose buffer for biological research. Search Slov. In H2O 48, 1357 (2019). Technol. Chim. Rawson, F.J., Cole, M.T., Hicks, J.M., Aylott, J.W., Milne, W.I., Collins, C.M., Jackson, S.K., Silman, N.J., Mendes, P.M.: Electrochemical communication with the inside of cells using micropatterned vertical carbon nanofibre electrodes. 38, 2874 (2004), Kiss, L., Kunsgi-Mt, S.: Solubility determination of hydroquinone in dichloromethane, trichloromethane and carbon tetrachloride by using the co-solvent calibration method.